Application of Adding VB12 to Diluent for the Preservation of Hu Sheep Semen

Abstract:

When storing sheep semen,adding a certain amount of vitamin B₁₂(VB₁₂) to the diluent is important in improving semen quality,maintaining sperm acrosome integrity,prolonging sperm in vitro survival time,and increasing sperm survival rate and motility.This study investigates the effect of different concentrations of VB₁₂ added to diluent on the preservation of Hu sheep semen.Ten Hu sheep were selected for the experiment,and semen was collected using the pseudo-vaginal method.Different concentrations of VB₁₂ diluent(0,0.025,0.05 and 0.075 mg/mL) were added and stored at 4 ℃.The research results showed that on the first and second day of storage,there was a significant difference in sperm abnormality rate between the VB₁₂ -added groups and the control group(P<0.05).The 0.05 mg/mL VB₁₂ group had significantly higher sperm survival rates and activity from day 2 to day 5 compared to other concentration groups other concentration groups(P<0.05),and the sperm abnormality rate on days 4 and 5 was significantly higher than in other groups(P<0.05).The sperm abnormality rate of all VB₁₂ addition groups on day 5 was significantly higher than that of the control group(P<0.05).In summary,adding an appropriate amount of VB₁₂ to the diluent can effectively improve the preservation effect of Hu sheep semen,prolong the survival rate and motility retention time of sperm,and to some extent reduce the sperm abnormality rate.This provides a basis for optimizing Hu sheep semen preservation technology and improving the success rate of artificial insemination.