Isolation,Identification of Pathogens,and Drug Sensitivity Testing in A Case of FootandMouth Abscess in Sheep

Abstract:

This study employed PCR molecular biology techniques and bacterial isolation identification methods to investigate skin tissue and purulent fluid samples from diseased sheep in a farm.The results indicated that:(1)None of the four scab samples tested positive for sheep pox,contagious ecthyma,or footandmouth disease virus;(2)Bacterial isolation and identification from the two samples of purulent fluid revealed the presence of Staphylococcus aureus,which was found to be sensitive to several antibiotics,but resistant to penicillin and ampicillin.The study concludes that the primary pathogenic bacteria in this case of sheep foot-and-mouth purulent abscess are Staphylococcus aureus,and it is recommended that the farm strengthen monitoring and prevention measures against this pathogen,while formulating a reasonable treatment plan based on the antibiotic sensitivity test results.