Abstract:To reveal the composition of the gut microbiota in wild boars from the southwestern region of Yunnan and to explore the impact of different geographical environments on their gut microbiota, a study was conducted from September to November 2023, collecting gastrointestinal tract tissues from wild boars in Lincang, Dehong, and Nujiang. After extracting DNA, 16S rDNA V3-V4 region amplicon sequencing and analysis were performed. The results showed that a total of 8,218 valid sequences were obtained from the wild boar samples, which were clustered into 8,218 operational taxonomic units (OTUs), belonging to 33 phyla and 554 genera. The Firmicutes and Bacteroidetes were the dominant phyla in all wild boar samples. The fecal microbial community structure and function of wild boars living in different regions differed. In summary, the gut microbiota of wild boars from the three regions share certain commonalities, but there are also significant differences. This indicates that different living environments can lead to variations in the gut microbiota of wild boars, and a more complex living environment can result in a richer gut microbial community.