Abstract:This study focused on the application of non-invasive transcervical embryo recovery technology in ewes,and evaluated the effect of different administration methods on the recovery efficiency.Cervical relaxation,expansion and uterine contraction in female sheep were achieved by injecting estradiol benzoate and oxytocin,creating ideal conditions for embryo recovery.From 6.5 to 7 days after estrus,embryos were collected by cervical surgery using standing stand and strict preoperative preparation.The experimental results showed that the embryo recovery rate (93.75% and 100%) and the fluid recovery efficiency (96.28% and 96.75%) achieved high success rates,and the average number of recovered embryos also showed good levels (5.0 and 5.6).This study provides powerful data for the transcervical non-surgical embryo recovery technology,and has