Abstract:In order to investigate the inhibitory effect of traditional Chinese medicine compound preparation on the proliferation of porcine transmissible gastroenteritis virus (TGEV) on PK-15 cells in vitro.In the experiment,four formulas of compound Chinese medicine preparations were selected,which were composed of seven traditional Chinese medicine extracts of Pulsatilla chinensis,Phellodendron chinense,Green husk,fried Atractylodes,jiao Sanxian,Herba agrimoniae,woody fragrance.Three modes of action were adopted:adding drugs first and then adding viruses,adding drugs and viruses after a period of time,adding viruses first and then adding drugs.The inhibitory adsorption and penetration of drugs on viruses,direct killing and inhibition of replication were observed.The results showed that the compound Chinese medicine preparation at a dose of 1 mg/mL had an effect on the proliferation of TGEV on PK-15 cells in vitro.The formula 2 was superior to the other three formulas,which had a good inhibitory effect on TGEV,and the direct killing effect had the best protective effect on cells.The cell survival rate was 75%(P<0.01),and an effective compound Chinese medicine preparation with three ways of antivirus was screened.The results can provide a scientific basis for the subsequent screening of antiTGEV drugs.
