Abstract:To establish an in vitro maturation and parthenogenetic activation culture system for Tan sheep oocytes,Tan sheep oocytes derived from ex vivo ovaries were subjected to in vitro maturation culture and parthenogenetic activation was performed using chemical activation method.The results showed that the average number of eggs obtained from detached ovaries of Tan sheep was 3.07,the maturation rate of oocytes was 54.78%,the cleavage rate was 61.92%,and the blastocyst rate was 17.72%.There was no significant difference between the replicates of the experiment(P>0.05).In summary,the culture system established in this experiment is relatively stable and suitable for the in vitro maturation culture of Tan sheep oocytes,laying a laboratory foundation for the production of in vitro fertilized embryos and cloned embryos.
