Abstract:Live animal transportation is a significant route for the spread of animal diseases,with most major outbreaks associated with longdistance transportation.The Revised Animal Epidemic Prevention Law of 2021 stipulates that “animals transported across provincial,autonomous regional,or directly governed municipal borders via road transport must pass through designated channels established by the respective provincial,autonomous regional or directly governed municipal governments when entering or transiting through the province”.Yunnan,renowned for its largescale livestock and poultry framing,experiences frequent livestock transportation activities.The Animal Designated Channel Inspection Station (hereinafter referred to as the inspection station),serves as the primary point for animal entry inspection into the province.The efficacy of the inspection station’s efforts in preventing and controlling major animal epidemics is of paramount importance.The Yunnan Provincial Animal Health Inspection and Quarantine Institute is the regulatory body overseeing the specific implementation of checkpoints within the province.In recent years,based on local conditions and despite constraints in personnel and funding,the institute has continuously explored new supervisory methods,achieving breakthroughs in the standardized construction of stations and innovative informationbased supervision models,effectively enhancing the operational of inspection stations.