Abstract:The aim of this study was to investigate the inhibitory effect of honokiol (HNK) on water fungus spores and to analyse its possible mechanism of action.In the experiment,drug media with different concentrations of HNK (512,256,128,64,32,16,8,4,2,1 mg/L) were prepared by double dilution method,and the recovered water fungus was inoculated on the media by rapeseed culture method,and the germination of water fungus spores was observed 24 h later to determine the inhibition concentration of HNK on water fungus spores.At the same time,the concentration of HNK with obvious inhibitory effect was selected,and the effect of HNK on the ultrastructure of water fungus spores was analysed by scanning electron microscope and transmission electron microscope respectively.The results showed that at the concentration of 48 mg/L,the morphological observation showed that after HNK treatment,the spore cells were relatively severely damaged and showed severe edema.The intracellular matrix was sparse and dissolved,and the organelles were swollen.Massive cell membrane disintegration and plasmic wall separation.The results of this study showed that HNK caused obvious damage to the intracellular structure of spore and inhibited the growth and germination of spore at appropriate concentration.
