Abstract:Based on the needs of "New Agriculture" animal production professional innovative compound animal husbandry talent training, CRISPR/Cas9mediated gene knockout mouse model is introduced into the experimental course of “Animal Molecular Genetic Markers”. The gene deletion molecular marker detection and analysis experiment was designed, and the PBLbased hybrid teaching mode is adopted, so that students can systematically master the relevant theories and experimental techniques such as genomic DNA extraction, PCR amplification technology, agarose gel electrophoresis analysis, and genotype determination. The organic combination of the practical links of multiple professional courses promotes the integration of students' theoretical knowledge and experimental skills, stimulates students' enthusiasm for learning, enhances professional identity, and cultivates comprehensive quality and innovation ability.
