Abstract:Riemerella anatipestifer (RA) is one of the common pathogenic bacteria in waterfowl, which causes high incidence rate and mortality after infecting the host. It is difficult to prevent and control, which seriously threatens the healthy development of poultry breeding industry. This study confirmed through the combination of clinical symptoms, pathological changes, and laboratory diagnosis that the pathogen causing the disease in duck flocks is Riemerella anatipestifer, and successfully isolated a strain of Riemerella anatipestifer HN-RA2023. Further analysis of 16S rRNA sequencing and phylogenetic tree revealed that the HN-RA2023 isolate shares a homology of 98.9% to 99.9% with the reference strain, and is located on the same branch as the SD-RA2018 strain. The results of this experiment will provide reference for the diagnosis and treatment of Riemerella anatipestifer in Hunan region。