Abstract:When storing sheep semen, adding a certain amount of vitamin B12 (VB12) to the diluent is important in improving semen quality, maintaining sperm acrosome integrity, prolonging sperm in vitro survival time, and increasing sperm survival rate and motility. This study investigates the effect of adding different concentrations of VB12 to diluent on the preservation of Hu sheep semen. Ten Hu sheep were selected for the experiment, and semen was collected using the pseudo-vaginal method. Different concentrations of VB12 diluent (0.00 mg/ml, 0.025 mg/ml, 0.05 mg/ml, 0.075 mg/ml) were added and stored at 4 ℃. The research results showed that on the first and second day of storage, there was a significant difference in sperm abnormality rate between the VB12-added groups and the control group (P<0.05). The sperm survival rate and motility of the VB12 group at 0.05 mg/ml on days 2 to 5 were significantly higher than those of other concentration groups (P<0.05), and the sperm abnormality rate on days 4 and 5 was significantly higher than that of different groups (P<0.05). The sperm abnormality rate of all VB12 added groups on day 5 was significantly higher than that of the control group (P<0.05). In summary, adding an appropriate amount of VB12 to the diluent can effectively improve the preservation effect of Hu sheep semen, prolong the survival rate and motility retention time of sperm, and to some extent reduce the sperm abnormality rate. This provides a basis for optimizing Hu sheep semen preservation technology and improving the success rate of artificial insemination.
