Abstract:The combination of live egg harvesting (OPU) and in vitro embryo production (IVP) technology can significantly improve the reproductive efficiency of cattle, which is of great significance for the expansion of the core population of beef cattle. In recent years, OPU/IVP technology has been rapidly promoted worldwide, gradually replacing in vivo embryo production technology. In vitro embryo production of beef cattle can not only produce a large number of high-quality embryos, but also significantly improve the dissemination efficiency of excellent genetic resources. Method: This experiment focuses on the application and analysis of OPU-IVF technology in the expansion of beef cattle core population. By screening 12 excellent donor cows of different age groups in the core population, ultrasound-guided follicular puncture (OPU) was performed three times in different seasons to extract oocytes. In vitro fertilization (IVF), in vitro culture, cryopreservation, and transplantation procedures were performed in a timely manner. The implementation of each stage was monitored and analyzed, and the application effect was statistically analyzed based on the statistical significance level of p<0.05. Result: The optimized OPU-IVF process can collect 17.6 eggs per head, achieve a cleavage rate of 69.6% and a blastocyst rate of 28.6% after in vitro fertilization, and obtain 3.5 OPU-IVF embryos per head; After embryo freezing and thawing, the pregnancy rate of transfer increased to 46%, which can effectively improve the reproductive efficiency of excellent core breeding cows and quickly solve problems such as a small number of excellent core breeding populations and insufficient supply of high-quality seed sources.
