Research Progress of Lytic Phage Endolysin and Holin Against Gramnegative Bacteria

Abstract:

With the increasing severity of bacterial drug resistance, people began to look for alternatives to antibiotics, and bacteriophage endolysin and holin attracted attention. Among them, endolysin show good antibacterial potential and have certain advantages compared with antibiotics and bacteriophages. The action characteristics of endolysin determine that it needs the cooperation of holin when it inhibits gramnegative bacteria. Therefore, it has been studied to use genetic engineering to recombine endolysin and perforin to solve this problem. In addition, in the study of bacteriophage bacteria interaction, previous studies have shown that bacteriophage genes are involved in the regulation of bacterial communication system, of which endolysin and holin are important regulatory factors. This article reviews the research progress of bacteriophages and bacteriophage preparations, the research progress and application of endolysin and holin against gramnegative bacteria, with a view to providing ideas for future research on bacteriophage lyase.