Abstract:As an important substance to regulate calcium and phosphorus metabolism and promote calcification in poultry, VD3 has been widely used in poultry feed. However, due to the impact of sunlight exposure, the amount of VD3 produced in animals is very limited, which is difficult to meet the needs of poultry during growth. Therefore, this paper studies the development of tibia and nutrient metabolism of broiler chickens with different levels of 25-OH- D3 and VD3 combined diets. The results showed that under the same nutrient level, the development of broiler tibia and nutrient metabolism in broilers were improved to varying degrees when supplemented with 25-OH-D3 69 μg/kg and VD31 380 IU/kg, but they were inhibited when supplemented in excess. This indicated that the appropriate addition of 25-OH-D3 and VD3 at the age of day can effectively improve the growth and development of broilers, which can lay a good foundation for improving production performance.