Abstract:This study was to verify the effects of different types and proportions of yeast culture (YC) on the rumen fermentation parameters of sheep in vitro. This experiment adopts a full factorial design, and different types and proportions of YC are added under the condition of fermentation substrate. The experiment was divided into a control group and 4 test groups and the concentrations of in vitro fermentation parameters were determined. It was found that YC-3 can significantly reduce the pH and propionic acid concentration of the culture medium, and significantly increase the isobutyric acid concentration and ethylene-propylene ratio. Adding 1% and 2% YC can significantly increase the concentration of acetic acid in the culture medium under in vitro conditions, and adding 4% YC can significantly reduce the concentration of isovaleric acid. The different sources and different levels of YC will affect the fermentation parameters such as pH, propionate and ethylenepropylene ratio of sheep rumen in vitro fermentation. These results are expected to be used to guide livestock production practice.