Abstract:The purpose of this experiment was to study the effect of adding different concentrations of soybean lecithin (SL) on the cryopreservation of semen in East Friensian milk sheep. We added 18% egg yolk to the basic dilution of Tris as the control group, and added 0.5%,1%,1.5%,2%,and 2.5% SL as the test group. We measured sperm motility and acrosome integrity after thawing frozen semen. The results showed that there were significant differences(P<0.05) in the sperm viability and acrosome integrity rate between the thawed semen diluted with 0.5% and 2.5% SL freezing diluent and other groups(P<0.05);adding 18% egg yolk and 1%~2% There was no significant difference between the sperm motility rate and acrosome integrity rate in semen diluted with SL frozen diluent after thawing(P>0.05); the semen diluted with 18% egg yolk and 1.0%~1.5% SL frozen diluent, after artificial insemination The pregnancy rate of ewes was not significantly different from that of the control group(P>0.05).Therefore,soybean lecithin can be used as a cryoprotectant for the cryopreservation of semen in East Friensian milk sheep,and the optimum concentration is 1%~2%(g/L).
