Abstract:Hemagglutination (HA) and hemagglutination inhibition test (HI) are mainly used for Newcastle disease pathogen identification and antibody titer determination. Revised and refined to make it more standard and normative. In order to understand the requirements of the new version of the standard as soon as possible, and master the essentials of operation, the HA and HI tests in the new and old versions of the standard were compared. The new version of the standard adds the calibration of the 4HAU antigen preparation of the HA and HI tests, and changes in the standing time and the judgment of the results. The new version of the standard is more precise and detailed in the operation steps, and the 4HAU antigen configuration is more accurate, which makes the HI test results more scientific. Combined with the actual test, this paper provides a reference for the majority of laboratory inspectors, college students and scientific researchers to better understand the new version of the standard and operate it more proficiently, thereby improving the accuracy of the test results.
