Specificity Test of the Universal RPA Detection Method for Sheep Pox Virus and Goat Pox Virus

Abstract:

In this study, the previously established general RPA detection method for sheep pox virus and goat pox virus was used to detect footandmouth disease virus, infectious pustular dermatitis virus, fowl pox virus, sheep pox virus, sheep pox vaccine and sheep pox recombinant plasmid samples. Based on this, we verified the specificity of the general RPA detection method for sheep pox virus and goat pox virus for the detection of sheep pox virus and goat pox virus. The results show that the method can show positive bands on the test strip for the positive samples of sheep pox vaccine, sheep pox recombinant plasmid, sheep pox virus nucleic acid and goat pox virus nucleic acid, but does not show positive bands for other samples. This shows that the method has good specificity and has broad prospects in the detection of sheep poxvirus and goat poxvirus.