Semen Collection Experiment of Tibetan Sheep by Electrical Stimulation in Alpine Pastoral Area of Gansu Province

Abstract:

This paper is to study whether the electric stimulation semen collection technology can be popularized and applied in the breeding of Tibetan sheep in the alpine pastoral area of Gansu Province and whether this technology has an impact on the semen quality and semen collection volume of rams. The research team of the project conducted an experiment on semen collection by electrical stimulation of Tibetan sheep breed rams in Danma Township, Tianzhu County, Gansu Province. In this paper, the Eulertype Tibetan sheep rams selected locally were selected, and the semen was collected twice a day during the mating period. On the first day (marked as d1), the traditional false vaginal semen collection method was used in the morning, and the electric stimulation method was used to collect semen in the afternoon. The next day (marked as d2) semen was collected by electrical stimulation in the morning and traditional false vaginal semen collection in the afternoon. We crosscollected semen with different semen collection methods in order to reduce errors, continuously collected semen for 6 days, and checked the quality of semen collection, semen density, and sperm motility in time. The experimental results show that the amount of semen collected by electrical stimulation of Tibetan sheep rams in Tianzhu pastoral area of Gansu Province is all between 0.80 mL and 0.96 mL. This is in line with the normal semen volume of Tibetan sheep, the average sperm motility is above 0.80, and the average semen density is above "medium". The quality of this semen meets the quality requirements of artificial insemination semen and can be applied to the practice of Tibetan sheep breeding.