Abstract:Neospora Caninum is an intracellular protozoan parasite that can cause abortion and neonatal fetal death in a variety of mammals, including sheep and goats. The purpose of this study was to estimate the prevalence of neosporoasis in scapegoat in Hejing County, Bayingolin Mongolian Autonomous Prefecture. PCR was used to amplify the NC5 gene fragment of neosporoasis canine, and some positive products were sequenced. Results Showed that 13 of 165 scapegoat (7.9%) were positive for canine N.caninum. Age, abortion history, dog ownership and infection rate were significantly different (P< 0.05). The infection rate of ewes over 2 years old was the highest. In addition, the infection rate of neosporoasis was higher in ewes with a history of abortion , and the presence of dogs around the breeding site also affected the infection rate, and the infection rate of sheep with dogs 11.4%, was higher than that of sheep without dogs. The partial sequence of NC5 gene was compared with that of NC5 gene stored in GenBank, and the homology of NC5 gene was 92%~100%. To our knowledge, this is the first phylogenetic analysis of N.caninum in scapegoat from Hejing county. This information is important for designing control schemes to reduce the economic losses of animal husbandry.
