Abstract:Severe infectious diseases such as highly pathogenic avian influenza (HPAI) and Newcastle disease (ND) are serious hazards to the poultry industry, and they are diseases that need to be prevented and controlled in the poultry industry. Among them, HPAI is a compulsory immunization disease stipulated by the state. Xinzhou District of Wuhan City is a large poultry breeding area, with more than 350 largescale poultry farms and a breeding scale of nearly 11.4 million birds. This article is to understand the pass rate of highly pathogenic avian influenza and Newcastle disease immune antibodies in largescale farms in Xinzhou District, Wuhan City in 2021. The Xinzhou District Agricultural Technology Extension Service Center of Wuhan City has conducted egg sample inspections on largescale chicken farms in the area for 12 consecutive months. Using hemagglutination and hemagglutination inhibition tests, the highly pathogenic avian influenza H5 subtype and H7 subtype were detected respectively. and Newcastle disease immune antibodies. The results showed that the qualified rates of overall immune antibodies against highly pathogenic avian influenza H5 subtype, H7 subtype and Newcastle disease were 94.1%, 96.4% and 88.9%, respectively. All of these have reached the standard of not less than 70.0% for the pass rate of immune antibodies stipulated by the Ministry of Agriculture and Rural Affairs.