Abstract:In order to understand the prevalence of Avian Leucosis in Tianshui City, Gansu Province, this paper used realtime fluorescent PCR method to carry out pathogenic detection on 480 cloacal swabs collected from 24 largescale chicken farms from 2020 to 2021. The results showed that 18 of the 24 scale farms were positive, with a positive rate of 73.33%. Among the 480 samples, 203 were positive, and the positive rate of the samples was 42.29%. Among them, the infection rate of Avian Leucosis for different purposes is different. Among the 360 laying hen samples, 138 were positive, and the positive rate was 38.33%. Among the 120 broiler samples, 65 were positive, with a positive rate of 54.17%. The results showed that Avian Leucosis virus was prevalent in largescale chicken farms in Tianshui City, and the infection rate of broiler chickens was higher than that of laying hens.