Abstract:Although the teaching practice of many courses proves that the MOOC can effectively improve the teaching effect, there is none report for teaching exploration and practice cases using MOOC in Biostatistics and Experimental Design. Here, based on the use of MOOC, a variety of teaching activities were carried out, such as realtime checkin, classroom test, random roll call, questionnaire survey and theme discussion, and we made a survey on whether these actions improved the teaching quality to provide reference for the teaching reform of "Biostatistics and Experimental Design" and improve the teaching quality.