稀释液中添加VB12在湖羊精液保存中的应用

Application of Adding VB12 to Diluent for the Preservation of Hu Sheep Semen

摘要:

羊精液保存时,稀释液中添加一定量的维生素B₁₂(VB₁₂),对提高精液品质具有重要作用,可维持精子顶体完整率,延长精子体外存活时间和提高精子存活率和活力。本研究旨在探究稀释液中添加不同浓度VB₁₂对湖羊精液保存效果的影响。试验选取10只湖羊,采用假阴道法采集精液,添加不同浓度的VB₁₂稀释液(0、0.025、0.05和0.075 mg/mL),在4 ℃条件下冷藏保存。研究结果表明:在保存初期第1、2天,各VB₁₂添加组精子畸形率与对照组差异显著(P<0.05)。0.05 mg/mL VB₁₂组第2~5天精子存活率和活力显著高于其他浓度组(P<0.05),第4、5天精子畸形率显著高于其他组(P<0.05),所有添加VB₁₂组第5天的精子畸形率均显著高于对照组(P<0.05)。综上所述,稀释液中添加适量的VB₁₂可以有效提高湖羊精液保存效果,延长精子的存活率和活力保持时间,并在一定程度上降低精子畸形率,为优化湖羊精液保存技术,提高人工授精成功率提供了依据。

Abstract:

When storing sheep semen,adding a certain amount of vitamin B₁₂(VB₁₂) to the diluent is important in improving semen quality,maintaining sperm acrosome integrity,prolonging sperm in vitro survival time,and increasing sperm survival rate and motility.This study investigates the effect of different concentrations of VB₁₂ added to diluent on the preservation of Hu sheep semen.Ten Hu sheep were selected for the experiment,and semen was collected using the pseudo-vaginal method.Different concentrations of VB₁₂ diluent(0,0.025,0.05 and 0.075 mg/mL) were added and stored at 4 ℃.The research results showed that on the first and second day of storage,there was a significant difference in sperm abnormality rate between the VB₁₂ -added groups and the control group(P<0.05).The 0.05 mg/mL VB₁₂ group had significantly higher sperm survival rates and activity from day 2 to day 5 compared to other concentration groups other concentration groups(P<0.05),and the sperm abnormality rate on days 4 and 5 was significantly higher than in other groups(P<0.05).The sperm abnormality rate of all VB₁₂ addition groups on day 5 was significantly higher than that of the control group(P<0.05).In summary,adding an appropriate amount of VB₁₂ to the diluent can effectively improve the preservation effect of Hu sheep semen,prolong the survival rate and motility retention time of sperm,and to some extent reduce the sperm abnormality rate.This provides a basis for optimizing Hu sheep semen preservation technology and improving the success rate of artificial insemination.