鸭疫里默氏杆菌病诊断及分型鉴定

Diagnosis and Genotyping of Riemerella Anatipestifer Infection

摘要:

鸭疫里默氏杆菌是水禽中常见的病原菌之一,其感染宿主后导致较高的发病率和死亡率,其防控难度大,严重威胁禽类养殖业的健康发展。本研究通过结合临床症状、病理变化和实验室诊断,证实引起鸭群发病的病原为鸭疫里默氏杆菌,并成功分离1株鸭疫里默氏杆菌HN-RA2023。进一步通过16S rRNA测序和系统进化树分析,发现HN-RA2023分离株与参考株的同源性为98.9%~99.9%,并且分离株与SD-RA2018株处于同一分支。本试验结果将为湖南地区鸭疫里默氏杆菌病的诊断与治疗提供参考。

Abstract:

Riemerella anatipestifer(RA) is one of the common pathogenic bacteria in waterfowl,which causes high incidence rate and mortality after infecting the host.It is difficult to prevent and control,which seriously threatens the healthy development of poultry breeding industry.This study confirmed that Riemerella anatipestifer was the causative agent of the outbreak in duck flocks through the combination of clinical symptoms,pathological changes,and laboratory diagnosis,and successfully isolated a strain of riemerella anatipestifer HN-RA2023.Further analysis of 16S rRNA sequencing and phylogenetic tree revealed that the HN-RA2023 isolate shares homology of 98.9% to 99.9% with the reference strain,and was located on the same branch as the SD-RA2018 strain.The results of this study will provide reference for the diagnosis and treatment of riemerella anatipestifer in Hunan province.