基于全基因组数据绘制全球绵羊LCORL基因SNP变异图谱

A SNPs Variation Map of LCORL Gene among Global Sheep Breeds Based on Wholegenome Resequencing

摘要:

LCORL基因是影响绵羊生长速度和体格大小的重要基因,全面绘制该基因遗传变异图谱有助于系统评估该基因与生长性状的相关性,并挖掘与生长相关的变异位点,然而尚无关于该基因单核苷酸多态性(singlenucleotidepolymorphisms,简称SNPs)在绵羊不同品种中系统分布特征的报道。本研究基于实验室收集的国内外27个品种1 615只羊的全基因组重测序数据,共筛选出4 777个高可信度SNPs,其中16个为编码区突变,包括10个错义突变和6个同义突变。此外,本研究发现其中一个错义突变(g.38075364G>A)在大体型的多浪羊中受到选择,推测该位点可能与绵羊生长性状有关。本研究绘制了绵羊LCORL基因的遗传变异图谱,为探索与绵羊生长性状相关的突变和遗传标记提供了宝贵的资源。

Abstract:

The LCORLgene is an important gene that influences the growth rate and body size of sheep.Constructing a comprehensive genetic variation map of this gene will help systematically assess its correlation with sheep growth traits and identify growth-related variation sites.However,no study has systematically screened the single nucleotide polymorphisms(SNPs) of this gene in different sheep breeds.In this study,based on whole-genome resequencing data from 1 615 sheep across 27 domestic and international breeds,4 777 high-confidence SNPs were identified,including 16 mutations in coding regions,with 10 missense mutations and 6 synonymous mutations.Additionally,this study found that one missense mutation(g.38075364G>A) is under selection in large-bodied Dorper sheep,suggesting that this site may be related to sheep growth traits.This study constructed a genetic variation map of the LCORL gene in sheep,providing valuable resources for exploring mutations and genetic markers related to sheep growth traits.