基于Agricultural Animal Omics Database(AAOD)数据库挖掘绵羊GDF9基因变异

Exploration of Mutations of Sheep GDF9 Gene based on Agricultural Animal Omics Database(AAOD) Database

摘要:

目前利用大数据和组学数据库提高绵羊繁殖性能成为解决当前产业发展的关键之一。绵羊生长分化因子9(GDF9)基因对卵泡发育、排卵率及产羔数起着至关重要的作用,也是目前确定的绵羊多胎性状的主效基因之一;虽然其部分重要突变位点与产羔数有关,但绵羊该基因所有可能基因变异如何挖掘仍然是一个尚待解决的问题。近年来,西北农林科技大学动物科技学院姜雨团队开发出Agricultural Animal Omics Database(AAOD)数据库,它整合了全球公共数据及中国本地特色绵羊品种的高深度基因组数据,涵盖50多个品种的遗传变异(SNPs、InDels)、表观标记及表型关联信息,并实现每月动态更新。基于此,本研究旨在利用AAOD数据库挖掘绵羊GDF9基因变异,并期望深入解析GDF基因的分子结构、表达模式、富集分析、挖掘新的与繁殖力相关的突变位点及其在绵羊繁殖力中的潜在作用。这些结果将为基因编辑技术改良绵羊繁殖力提供新的潜在位点,将为提高绵羊繁殖力提供了遗传改良的理论依据与策略。

Abstract:

Currently,the application of big data and omics databases has become crucial for enhancing sheep reproductive performance in industry development.The Growth Differentiation Factor 9(GDF9) gene plays a vital role in follicular development,ovulation rate,and lambing number,serving as one of the major genes determining sheep prolificacy.Although some key mutation sites are associated with lambing numbers,identifying all potential genetic variations of GDF9 in sheep remains unresolved.In recent years,Prof.Jiang Yu’s team at Northwest A&F University developed the Agricultural Animal Omics Database(AAOD),which integrates global public data and high-depth genomic information from Chinese indigenous sheep breeds.This database contains genetic variants(SNPs and InDels),epigenetic markers,and phenotype association data across over 50 breeds,with monthly updates.Based on AAOD,this study aimed to mine GDF9 gene variants in sheep and systematically investigate its molecular structure,expression patterns,enrichment analysis,and novel fertility-related mutations.These findings will identify novel potential functional sites of GDF9 in sheep reproduction,providing new targets for gene editing technologies and establishing a theoretical foundation for genetic improvement strategies to enhance sheep reproductive efficiency.