岔口驿马弓形虫病血清抗体检测

Serological Antibody Testing for Toxoplasmosis in Chakouyi Horse

摘要:

为加强畜禽资源保护和岔口驿马产业的快速发展,为马病防治提供技术指导。在天祝县全县范围内开展马弓形虫病检测工作。马静脉采血后,离心分离出待检血清,采用间接血凝试验(IHAT)对马血清中弓形虫感染抗体进行定性和定量检测。结果表明:抽检12个乡镇240份马血样,检测结果抗体阳性9份,阳性率3.75%。其中祁连镇抽样20份,抗体阳性5份,阳性率达25.0%。240份检样中,血清抗体滴度0、1∶4、1∶16和1∶64分别为88、78、65、9个样品。检测结果表明,多年来天祝县马弓形虫病一直在可控安全范围内在我县马属动物间流行,未对天祝县马产业发展产生严重影响,但此项检测工作仍是对天祝县人兽共患传染病防控工作内容的重要补充。

Abstract:

In order to strengthen the protection of livestock and poultry resources and promote the rapid development of Chakouyi horse industry,and provide technical guidance for the prevention and control of horse disease.As one of the important contents of the general survey of equine protozoonosis,the detection of equine toxoplasmosis was carried out in the whole county.Blood samples were collected from horse veins,and the serum was centrifuged.Indirect hemagglutination test(IHAT) was used to detect the Toxoplasma gondii infection antibody in horse serum qualitatively and quantitatively.240 horse blood samples were sampled from 12 towns.The results showed that 9 samples were positive for antibodies,with a positive rate of 3.75%.Among these,20 samples were collected from Qilian town,5 of which were positive,resulting in a positivity rate of 25.0%.In the 240 samples tested,the serum antibody titers of 0,1∶4,1∶16 and 1∶64 were 88,78,65 and 9 samples respectively.The test results show that toxoplasmosis in horse in Tianzhu county has been epidemic among equine animals within the controllable and safe range over many years,which has not had a serious impact on the development of horse industry in Tianzhu county.However,this testing work remains an important supplement to the prevention and control of zoonotic infectious diseases in Tianzhu county.