青海甘德一起犊牛病毒性腹泻病原检测分析

Detection and Analysis of the Pathogen of Viral Diarrhoea in Calves in Gande,Qinghai

摘要:

2024年4~6月份,青海甘德某牦牛养殖合作社的犊牛出现精神沉郁、消瘦、腹泻等临床症状。为检测病原探明发病原因,采集腹泻犊牛肛拭子、粪便等样品10份,采用PCR方法检测牛冠状病毒、牛轮状病毒、牛病毒性腹泻病毒、牛腺病毒、牛肠道病毒、牛环曲病毒、牛诺如病毒、牛纽布病毒、球虫、隐孢子虫和贾第鞭毛虫。结果表明,在10份腹泻粪样中,均仅检出牛冠状病毒,其他病原未检出。这说明该合作社犊牛腹泻由牛冠状病毒感染引起。在犊牛发生腹泻后,可通过静脉注射补液,利用中药强心剂强心,同时使用适当的抗菌素进行预防细菌性病原继发感染,应加强营养管理,增强免疫力,减少环境应激,注意圈舍消杀,减少污染环境暴露及激发感染,可达到有效地治疗与防控目的,本研究为该合作社犊牛腹泻防治提供一定的参考。

Abstract:

From April to June 2024,calves at a yak farming cooperative in Gande,Qinghai,showed clinical symptoms such as depression,weight loss,and diarrhea.To confirm the causes of the disease,10 samples,including anal swabs and feces from diarrhetic calves,were collected.PCR method was used to detect bovine coronavirus,bovine rotavirus,bovine viral diarrhoea virus,bovine adenovirus,bovine enterovirus,bovine torovirus,bovine norovirus,bovine nebovirus,Eimeria spp.,Cryptosporidium spp.,and Giardia.The results showed that bovine coronavirus was detected in all 10 diarrheic fecal samples,while no other pathogens were found.The results indicated that the diarrhoea in the calves at the cooperative was caused by bovine coronavirus infection.After diarrhoea occuring in calves,rehydration fluid can be injected intravenously,the use of traditional Chinese medicine to strengthen the heart,while the use of appropriate antibiotic for the prevention of bacterial pathogens secondary infections,nutritional management should be strengthened,immunity should be enhanced,environmental stress should be reduced,and attention should be paid to disinfestation of pens to reduce exposure to contaminated environments,which can be effective in the treatment and prevention of the purpose of the control.The present study provides a references for the prevention and control of diarrhoea in calves in this cooperative.