1例羊口蹄脓包病例病原菌分离鉴定及药敏试验分析

Isolation,Identification of Pathogens,and Drug Sensitivity Testing in A Case of FootandMouth Abscess in Sheep

摘要:

本研究采用PCR分子生物学技术和细菌分离鉴定的方法,对某养殖场病羊的皮肤组织和脓液的样本进行研究。结果表明:(1)四份痂皮样品均未检出羊痘、羊口疮及羊口蹄疫病毒;(2)两份口蹄脓液的细菌分离鉴定结果为金黄色葡萄球菌,该菌株对多种药物敏感,但对青霉素和氨苄西林不敏感。研究认为,本例羊口蹄脓包病例的主要致病菌为金黄色葡萄球菌,建议该养殖场加强对该病原菌的监测和防控,并根据药敏试验结果制定合理的治疗方案。

Abstract:

This study employed PCR molecular biology techniques and bacterial isolation identification methods to investigate skin tissue and purulent fluid samples from diseased sheep in a farm.The results indicated that:(1)None of the four scab samples tested positive for sheep pox,contagious ecthyma,or footandmouth disease virus;(2)Bacterial isolation and identification from the two samples of purulent fluid revealed the presence of Staphylococcus aureus,which was found to be sensitive to several antibiotics,but resistant to penicillin and ampicillin.The study concludes that the primary pathogenic bacteria in this case of sheep foot-and-mouth purulent abscess are Staphylococcus aureus,and it is recommended that the farm strengthen monitoring and prevention measures against this pathogen,while formulating a reasonable treatment plan based on the antibiotic sensitivity test results.