Abstract:With the rapid development of the sheep farming industry, enhancing the reproductive performance of sheep has become one of the key factors in addressing the increasing demand for meat production.The sheep growth differentiation factor 9 (GDF9) gene, as one of the important genes regulating multiparity, plays a crucial role in follicle development, ovulation rate, and lambing rate, and it has high conservation among other species;Partial important single nucleotide polymorphisms (SNPs) and insertion deletion mutations (InDels) of this gene have been identified,Among them, mutation sites such as FecGH, FecGT, and FecGV are related to litter size, providing new possibilities for gene editing technology to improve sheep fertility.Therefore, this study is based on the Animal Omics Database (AAOD) database developed by Jiang Yu"s team from the School of Animal Science and Technology at Northwest A F University, to explore the genetic variations of the sheep GDF9 gene,and it is expected to further analyze the molecular structure, expression pattern, enrichment analysis, and explore new mutation sites related to reproductive ability of GDF9 gene, as well as its potential role in sheep reproductive ability.These results provide a theoretical basis and strategy for genetic improvement to improve sheep reproductive capacity, and offer a new perspective for the study of livestock reproductive capacity.
