奶山羊胚胎无创回收技术的研究与应用探索

Research and Application Exploration of Non-invasive Recovery Technology of Dairy Goat Embryos

摘要:

本研究评估了不同给药途径(静脉注射与阴道内注射)对奶山羊胚胎无创回收效率的影响。通过注射苯甲酸雌二醇和催产素,成功促进了母羊子宫颈的松弛、扩张和子宫收缩,为胚胎回收创造了理想条件。在发情后6.5至7 d,采用经宫颈手术方式收集胚胎,并通过站立式保定架和严格的术前准备确保手术安全。实验结果显示,两种给药途径均表现出较高的胚胎回收率(平均93.75%至100%)和液体回收效率(平均96.28%至96.75%),同时回收的胚胎数量平均达到5.0至5.6枚,显示出良好的胚胎质量和数量。值得注意的是,阴道给药组取得了100%的回收率,显著优于静脉给药组。本研究为非手术经宫颈胚胎回收技术提供了有力数据支持,为畜牧业高效、安全的胚胎回收提供了重要参考,有助于推动畜牧业的持续发展和进步。

Abstract:

This study focused on the application of non-invasive transcervical embryo recovery technology in ewes,and evaluated the effect of different administration methods on the recovery efficiency.Cervical relaxation,expansion and uterine contraction in female sheep were achieved by injecting estradiol benzoate and oxytocin,creating ideal conditions for embryo recovery.From 6.5 to 7 days after estrus,embryos were collected by cervical surgery using standing stand and strict preoperative preparation.The experimental results showed that the embryo recovery rate (93.75% and 100%) and the fluid recovery efficiency (96.28% and 96.75%) achieved high success rates,and the average number of recovered embryos also showed good levels (5.0 and 5.6).This study provides powerful data for the transcervical non-surgical embryo recovery technology,and has