Excellent livestock and poultry germplasm resources are the material basis of sustainable development of the country and the basic guarantee of social development.China has very rich sheep breed resources,these precious germplasm resources for sheep genetic breeding provided sufficient material basis.In order to obtain more excellent sheep breeds,it is urgent to use biotechnology to further improve the sheep breeding system in China.This paper mainly introduces the concept of embryo engineering,genetic engineering and molecular engineering,and summarizes their application research and existing problems in sheep breeding in recent years,which provides a new technical way for breeding excellent sheep breeds and provides certain resource guarantee for the rapid development of China's sheep breeding industry.