复方中药制剂对猪传染性胃肠炎病毒的体外作用研究

Study on the Effect of Compound Chinese Medicine Preparation on Porcine Transmissible Gastroenteritis Virus in Vitro

摘要:

为了探讨中药复方制剂对猪传染性胃肠炎病毒(TGEV)在PK-15细胞上增殖的体外抑制作用。试验选择由白头翁、黄柏、青皮、炒白术、焦三仙、仙鹤草、木香7味中药提取物组成4个配方的复方中药制剂,采用同时给药、预防给药、治疗给药三种作用方式,观察药物对病毒的抑制吸附穿入、直接杀灭和抑制复制作用。结果显示:以1 mg/mL剂量的复方中药制剂在体外对TGEV在PK-15细胞上增殖影响,配方2优于其他3个配方,对TGEV有较好抑制作用,且直接杀灭作用对细胞的保护效果最好,细胞存活率达75%(P<0.01),并筛选出了同时具有3种方式抗病毒的有效复方中药制剂。该研究结果可为后续抗TGEV药物的筛选提供科学依据。

Abstract:

In order to investigate the inhibitory effect of traditional Chinese medicine compound preparation on the proliferation of porcine transmissible gastroenteritis virus (TGEV) on PK-15 cells in vitro.In the experiment,four formulas of compound Chinese medicine preparations were selected,which were composed of seven traditional Chinese medicine extracts of Pulsatilla chinensis,Phellodendron chinense,Green husk,fried Atractylodes,jiao Sanxian,Herba agrimoniae,woody fragrance.Three modes of action were adopted:adding drugs first and then adding viruses,adding drugs and viruses after a period of time,adding viruses first and then adding drugs.The inhibitory adsorption and penetration of drugs on viruses,direct killing and inhibition of replication were observed.The results showed that the compound Chinese medicine preparation at a dose of 1 mg/mL had an effect on the proliferation of TGEV on PK-15 cells in vitro.The formula 2 was superior to the other three formulas,which had a good inhibitory effect on TGEV,and the direct killing effect had the best protective effect on cells.The cell survival rate was 75%(P<0.01),and an effective compound Chinese medicine preparation with three ways of antivirus was screened.The results can provide a scientific basis for the subsequent screening of antiTGEV drugs.