滩羊卵母细胞体外成熟和孤雌激活试验

In Vitro Maturation and Parthenogenetic Activation Test of Tan Sheep Oocytes

摘要:

为建立滩羊卵母细胞体外成熟和孤雌激活培养体系,对离体卵巢来源的滩羊卵母细胞进行体外成熟培养,并用化学激活法进行孤雌激活。结果显示:滩羊离体卵巢平均得卵数为3.07枚,卵母细胞成熟率为54.78%,卵裂率为61.92%,囊胚率为17.72%,试验各重复之间差异不显著(P>0.05)。研究认为,本试验建立的培养体系较稳定,适合滩羊卵母细胞体外成熟培养,为体外受精胚和克隆胚生产奠定实验基础。

Abstract:

To establish an in vitro maturation and parthenogenetic activation culture system for Tan sheep oocytes,Tan sheep oocytes derived from ex vivo ovaries were subjected to in vitro maturation culture and parthenogenetic activation was performed using chemical activation method.The results showed that the average number of eggs obtained from detached ovaries of Tan sheep was 3.07,the maturation rate of oocytes was 54.78%,the cleavage rate was 61.92%,and the blastocyst rate was 17.72%.There was no significant difference between the replicates of the experiment(P>0.05).In summary,the culture system established in this experiment is relatively stable and suitable for the in vitro maturation culture of Tan sheep oocytes,laying a laboratory foundation for the production of in vitro fertilized embryos and cloned embryos.