6种布鲁氏菌病诊断试剂检测结果的比较

Comparison of Detection Results of 6 Kinds of Diagnostic Reagents for Brucellosis

摘要:

为比较不同布鲁氏菌病诊断试剂在实验室检测工作中性能的差异,本研究以96份奶牛血清为实验材料,采用虎红平板凝集试验(RBT)、试管凝集试验(SAT)、间接酶联免疫吸附试验(iELISA)、竞争酶联免疫吸附试验(cELISA)4种方法、6种不同试剂进行比对检测,将SAT结果作为金标准,分析其余5种诊断试剂的敏感性、特异性和符合率,并分析同种方法不同试剂检测结果的一致性。结果显示:(1)SAT检测出阳性样本3份,阴性样本93份。(2)与SAT结果相比,RBT、iELISAA和iELISAB的敏感性最高(100.00%),RBT和cELISAC的特异性最高(98.92%)。(3)RBT、iELISAA、cELISAC的符合率均在91.67%以上。(4)iELISAA与iELISAB之间,cELISAC与cELISAD之间一致性较差。研究表明:相比iELISAB和cELISAD试剂盒,iELISAA和cELISAC试剂盒的结果更加可靠;在开展布鲁氏菌病大规模初筛时宜选用敏感性高的试剂,如RBT和iELISAA,确诊时宜选用特异性高的试剂,如SAT和cELISAC。本研究结果为诊断试剂的选择提供参考,以提高诊断准确性。

Abstract:

In order to compare the performance of different brucellosis diagnostic reagents in laboratory tests,In this study,96 samples of cow serum were detected by 4 methods,including rose bengal plate agglutination test (RBT),serum agglutination test (SAT),indirect enzymelinked immunosorbent assay (iELISA) and competitive enzymelinked immunosorbent assay (cELISA),and 6 different reagents.SAT results were used as the gold standard.The sensitivity,specificity and coincidence rate of the other 5 diagnostic reagents were analyzed,and the consistency of the detection results of different reagents with the same method was analyzed.The results showed that 3 positive samples and 93 negative samples were detected by SAT.RBT,iELISAA and iELISAB showed the highest sensitivity (100.00%) compared with SAT.The specificity of RBT and cELISAC was the highest (98.92%).The coincidence rates of RBT,iELISAA and cELISAC were all above 91.67%.The consistency between iELISAA and iELISAB and between cELISAC and cELISAD was poor.The results of this study provided reference for the selection of diagnostic reagents to improve the diagnostic accuracy.