和厚朴酚对水霉孢子抑制效果的研究

Study on Inhibitory Effect of Honokiol on Water Fungus Spores

摘要:

本研究旨在探究和厚朴酚(honokiol,HNK)对水霉孢子的抑制效果并分析其可能的作用机制。试验采用二倍比稀释法制备不同HNK浓度的药物培养基(512、256、128、64、32、16、8、4、2、1 mg/L),利用油菜籽培养法将复壮后的水霉接种至培养基上,24 h后观察水霉孢子的萌发情况从而确定HNK对水霉孢子的抑制浓度。同时,选取其中抑制效果明显的浓度,分别利用扫描电镜和透射电镜观察、分析HNK对水酶孢子超微结构的影响。结果显示,48 mg/L浓度下,形态观察显示HNK处理后的水霉孢子细胞损伤相对严重,呈现重度水肿现象;细胞内基质稀疏、溶解,细胞器肿胀;细胞膜大面积崩解并质壁分离。本研究结果表明,在适宜浓度条件下,HNK对水霉孢子胞内结构造成了明显损伤,从而对其生长萌发具有显著的抑制效果。

Abstract:

The aim of this study was to investigate the inhibitory effect of honokiol (HNK) on water fungus spores and to analyse its possible mechanism of action.In the experiment,drug media with different concentrations of HNK (512,256,128,64,32,16,8,4,2,1 mg/L) were prepared by double dilution method,and the recovered water fungus was inoculated on the media by rapeseed culture method,and the germination of water fungus spores was observed 24 h later to determine the inhibition concentration of HNK on water fungus spores.At the same time,the concentration of HNK with obvious inhibitory effect was selected,and the effect of HNK on the ultrastructure of water fungus spores was analysed by scanning electron microscope and transmission electron microscope respectively.The results showed that at the concentration of 48 mg/L,the morphological observation showed that after HNK treatment,the spore cells were relatively severely damaged and showed severe edema.The intracellular matrix was sparse and dissolved,and the organelles were swollen.Massive cell membrane disintegration and plasmic wall separation.The results of this study showed that HNK caused obvious damage to the intracellular structure of spore and inhibited the growth and germination of spore at appropriate concentration.