《动物分子遗传标记》实验教学设计与实践

Experimental Teaching Design and Practice of "Animal Molecular Genetic Markers"

摘要:

基于“新农科”动物生产类专业创新型复合畜牧人才培养的需求，将CRISPR/Cas9介导的基因敲除小鼠模型引入动物分子遗传标记实验课程中，设计了基因缺失分子标记检测与分析实验，采用基于PBL的混合式教学模式，学生能系统掌握基因组DNA提取、PCR扩增技术、琼脂糖凝胶电泳分析、基因型判定等相关理论和实验技术，将多门专业课程的实践环节有机结合，促进了学生理论知识与实验技能的融会贯通，激发了学生的学习热情，提升了专业认同感，培养了综合素质和创新能力。

Abstract:

Based on the needs of "New Agriculture" animal production professional innovative compound animal husbandry talent training, CRISPR/Cas9mediated gene knockout mouse model is introduced into the experimental course of “Animal Molecular Genetic Markers”. The gene deletion molecular marker detection and analysis experiment was designed, and the PBLbased hybrid teaching mode is adopted, so that students can systematically master the relevant theories and experimental techniques such as genomic DNA extraction, PCR amplification technology, agarose gel electrophoresis analysis, and genotype determination. The organic combination of the practical links of multiple professional courses promotes the integration of students' theoretical knowledge and experimental skills, stimulates students' enthusiasm for learning, enhances professional identity, and cultivates comprehensive quality and innovation ability.