一例育成期比格犬皮下肿瘤的组织病理染色分析

Histopathological staining analysis of a case of subcutaneous tumor in beagle during breeding period

摘要:

犬皮肤肿瘤的发病常受到犬的品种和年龄影响，常见于老龄犬而鲜见于育成犬，尤其少见于育成期的比格犬。本文报告了一例对育成期比格犬自发性肿瘤的病例，并对其进行组织采样、病理染色分析和鉴别，为相关的疾病诊断提供参考。肿瘤本实验通过对一例8月龄比格犬的肿瘤组织采样进行组织苏木精-伊红染色和免疫组织化学染色。组织病理学检查发现，犬肿瘤组织位于真皮层，呈局灶性分布，内含大量嗜酸性胞质，细胞核形态异常。免疫组织化学结果表明，组织Vimentin和CK阳性，而α-SMA、KI67、S-100和CD34、均为阴性。综上，结合病理染色结果分析，初步判定可能是纤维组织细胞瘤。 本次病例报告为日后的犬肿瘤疾病诊断提供依据，也为实验用比格犬的质量控制工作提供新的视角。

Abstract:

The incidence of skin tumors in canine is often influenced by breed and age. They are more common in elderly and rarely seen in growing dogs, especially in growing Beagles. This article reports a case of spontaneous tumor in a beagle during the growing period, and conducts tissue sampling, pathological staining analysis and identification on it. Hematoxylin-eosin staining of the tumor tissue revealed that the canine tumor was located in the dermis, distributed focally, and contained a large amount of eosinophilic cytoplasm with abnormal nuclear morphology. Immunohistochemical results showed that Vimentin and CK were positive, while α-SMA, KI67, S-100 and CD34 were negative. Combined with the analysis of pathological staining results, the preliminary conclusion was probably fibrous histiocytoma. This case report provides a basis for the future diagnosis of canine tumor disease and a new perspective for the quality control of beagle.