Abstract:This study aims to understand the prevalence of porcine reproductive and respiratory syndrome (PRRS) in Jiuquan City. This experiment collected 638 samples from five agricultural counties and cities in Jiuquan City, and used reverse transcriptionpolymerase chain reaction (fluorescence RTPCR) method for nucleic acid detection. We analyze the prevalence in different regions, different growth periods, and different feeding modes. The results showed that a total of 29 PRRS nucleic acid positive samples were detected among 638 samples, and the PRRS positive infection rate was 4.55%. The PRRS positive infection rate in different regions ranges from 0-7.95%. The PRRS positive infection rates in slaughterhouses, largescale farms, and freerange households were 0, 2.96, and 6.82% respectively. The PRRS positive infection rates of breeding boars, fattening pigs, piglets, and sows were 0, 3.1, 5.38, and 6.36% respectively. The results showed that PRRS was sporadic in Jiuquan City. Largescale farms are highly standardized and have advanced feeding and management models with low PRRS positive infection rates. Piglets and sows are more susceptible to this disease. This test will provide technical support for the comprehensive prevention and control of PRRS in our city.