Abstract:This study used Cryptosporidium spp. 18S rRNA as the target gene, and found that 18 of the 101 fresh fecal samples collected were positive through nested PCR detection. The Cryptosporidium infection rates in sheep farms in different areas were 36% (18/50) in Liji Town, 0% (0/30) in Xin'an Town, and 0% (0/21) in Duigogang Town. No Cryptosporidium was detected. However, through the analysis of 4 sheep farms, it was found that 2 sheep farms (50%) were positive for Cryptosporidium infection, and the infection rates of different sheep farms were significantly different. Therefore, the infection rate of Cryptosporidium was simply evaluated by region, is debatable. The infection rate of Cryptosporidium parvum in goats was 33.3%, and the infection rate of Cryptosporidium parvum in Hu sheep was 2%. The infection rate of Cryptosporidium parvum in fattening sheep aged 2 to 6 months was 36%, and that of growing sheep aged 6 to 10 months (0%). The Cryptosporidium 18S rRNA gene fragment sequence analysis was performed on the samples that tested positive. We found that all 18 samples were Cryptosporidium xiaoi and there was no Cryptosporidium ubiquitum. In 1 goat farm and 1 Yanghu sheep farm that tested positive for Cryptosporidium infection, there was Cryptosporidium parvum infection, but there was no Cryptosporidium ubiquiti, and no Cryptosporidium parvum and Cryptosporidium ubiquita were found. of mixed infections. Current data suggest that Cryptosporidium shooni has a higher infection rate in goats aged 2 to 6 months (33.3%) and Hu sheep (2%). These 18 samples came from two largescale sheep farms, belonging to two sheep farms in Liji Town.
