云南大理地区猪繁殖与呼吸综合征病毒流行株遗传进化特征

Molecular Epidemiological Characteristics of Porcine Reproductive and Respiratory Syndrome in Dali

摘要:

[目的] 摸清大理地区猪繁殖与呼吸综合征流行情况,避免疫苗与流行毒株不匹配,影响防控效果；[方法] 在大理州范围内采集猪肺脏、脾脏4800份,通过试剂盒检测及自建PCR方法,经RNA提取和浓度检测、反转录、特异基因表达、琼脂糖凝胶电泳检测、PCR产物DNA测序、毒株序列进化树分析等步骤；[结果]有5份样品为阳性、其余4795份样品均为阴性,阳性率仅为0.104%,阳性样品均为高致病性PRRSV感染,经Nsp2基因测序、序列比对和遗传进化树分析,5份样品均属于JXA1毒株。[结论]大理地区PRRSV流行毒株是JXA1毒株,目前防控效果良好。由于变异毒株不断出现,市售商品化检测试剂存在漏检,应基于分子流行病学信息建立特异检测方法,避免监测工作中的漏诊。此外,科学合理选择使用疫苗防控,严格做好生物安全措施和生猪引种调运监管工作。

Abstract:

[Objective] To find out the prevalence of porcine reproductive and respiratory syndrome in Dali, avoid the mismatch between vaccines and epidemic strains, which will affect the prevention and control effect. [Method] 4800 pig lungs and spleens samples were collected in Dali Prefecture, perform RT-PCR detection through RNA extraction and concentration detection, reverse transcription, specific gene expression, agarose gel electrophoresis detection, PCR product DNA sequencing, and strain sequence evolution tree analysis. [Result] 5 samples were positive, the rest 4,795 samples were all negative, with a positive rate of only 0.104%. The 5 positive samples detected highly pathogenic PRRSV. After Nsp2 gene sequencing, sequence alignment and evolutionary tree analysis, 5 samples all belonged to the JXA1 strain. [Conclusion] At present, the PRRSV strain in Dali Prefecture is the JXA1 strain, and the prevention and control effect is significant. But we still need further strengthen the detection of PRRSV, scientifica