深度测序技术在畜禽疫病诊断中的应用

Application of Deep Sequencing Technology in Diagnosis of Livestock and Poultry Diseases

摘要:

下一代测序(NGS)，也被称为深度、高通量或大规模并行测序，可一次同时测定几十万到几百万条核酸分子序列。在畜禽疫病中应用于复杂诊断和密集监测、病因学、基因组学、进化和流行病学，以及宿主-病原体相互作用和感染生物学等方面。本综述首先简要介绍了深度测序技术，并通过实例对深度测序在畜禽疫病诊断中的应用进展进行阐述，为今后的相关研究提供些许参考。

Abstract:

Next generation sequencing (NGS), which is also known as deep, highthroughput or massively parallel sequencing. It can simultaneously determine the sequences of hundreds of thousands to millions of nucleic acid molecules at one time. Applications in livestock and poultry diseases include complex diagnosis and intensive surveillance, etiology, genomics, evolution and epidemiology, as well as hostpathogen interactions and infection biology. This review first briefly introduces deep sequencing technology, and explains the application progress of deep sequencing in the diagnosis of livestock and poultry diseases through examples. This provides some reference for future related research.