Abstract:In this study, six alfalfa (Medicago sativa) varieties including Gannong 3, Suntory, Handi, WL343, Juneng 601, and Weishen were tested for rhizobia inoculation. We use overwintering rate, number of branches, plant height, hay yield, crude protein, acid detergent fiber, neutral detergent fiber, relative feeding value, etc. as evaluation indicators. We used the gray relational method to analyze and evaluate the effect of rhizobia inoculation on the performance and nutritional value of alfalfa. The results showed that the inoculation of rhizobia could significantly improve the overwintering rate, plant height, hay yield and crude protein content of alfalfa varieties (P< 0.05). This had no significant effect on the number of branches, acid detergent fiber, neutral detergent fiber, and relative feeding value of alfalfa (P> 0.05). This can promote early tumor inoculation of alfalfa, and promote the improvement of alfalfa yield and forage quality. This is an effective measure to improve the grass yield and forage quality of alfalfa, and can be popularized and applied in a large area.