奶山羊布鲁氏菌病抗原与抗体双检测的效果分析

Analysis on the Effect of Double Detection of Antigen and Antibody of Brucellosis in Milk Goats

摘要:

为了有效检测净化奶山羊布鲁氏菌病群体，加快奶山羊产业健康稳步发展。本文采用布鲁氏菌病抗原与抗体双检测方法对陕西某奶山羊场进行了检测分析。结果表明，在某奶山羊场引入羊时，采用抗体单一检测发现全群228只羊感染了36只，感染率达到15.79%，发现感染后对本群感染羊进行了无害化处理和圈舍清洗消毒，3个月后对剩余的192只羊有进行了一次全群检测，结果检测出18只阳性羊，与上一次的净化方式一样处理后，同样又在3个月后对剩余的174只羊采用抗原与抗体双检测方法，布病抗体检测卡检测出8只羊，布病抗原检测卡检测出10只羊，而且用布病抗体检测卡和布病抗原检测卡检测出来的羊只编号完全不同，3个月后对剩余的156只羊进行同样的方法进行检测，结果仅检查出1只阳性羊，6个月后对剩余的155只羊进行同样的方法进行检测，未发现布病阳性羊只。利用抗原与抗体双检测继续在3个月、6个月进行检测均未发现阳性羊只。本试验得出结论，相比于抗体单一的布鲁氏菌病检测法，抗原抗体共同使用的检测方法布病阳性率明显的降低，且能短期内达到预期净化的效果，说明双抗使用在羊布鲁氏菌病检测中效果更好，更具有推广使用的意义。

Abstract:

In order to effectively detect and purify the brucellosis population of dairy goats and accelerate the healthy and steady development of the dairy goat industry, this paper used the dual detection method of brucellosis antigen and antibody to detect and analyze a dairy goat farm in Shaanxi. The results showed that when sheep were introduced into a dairy goat farm, 36 of the 228 sheep in the whole flock were infected by single antibody detection, and the infection rate reached 15.79%. disinfect. Three months later, a wholegroup test was carried out on the remaining 192 sheep, and 18 positive sheep were detected. After the same purification method as last time, the antigen and antibody double detection method was used for the remaining 174 sheep 3 months later. Eight sheep were detected by the brucellosis antibody detection card, and 8 sheep were detected by the brucellosis antigen detection card. 10 sheep, and the numbers of the sheep detected by the brucellosis antibody test card and the brucellosis antigen test card are completely different. Three months later, the remaining 156 sheep were tested by the same method, and only one positive sheep was detected. After 6 months, the remaining 155 sheep were tested by the same method, and no brucellosispositive sheep were found. The use of antigen and antibody double detection continued to detect positive sheep at 3 months and 6 months. The conclusion of this experiment is that compared with the brucellosis detection method with a single antibody, the detection method using both antigen and antibody has a significantly lower positive rate of brucellosis, and can achieve the expected purification effect in a short period of time. This shows that the use of double antibodies is more effective in the detection of sheep brucellosis, and it has more significance for popularization.