蜂毒素—死亡素重组抗菌肽的小鼠毒性试验

Toxicity Test of Melittin-Thanatin Recombinant Antimicrobial Peptide in Mice

摘要:

本试验旨在探索蜂毒素—死亡素重组抗菌肽（MT-W）对昆明小鼠的毒性影响。试验通过小鼠口服急性毒性试验和口服生物利用度试验，研究了MT-W对小鼠主要脏器的影响，并检测了血液中碱性磷酸酶、丙氨酸氨基转移酶、天冬氨酸氨基转移酶等生化指标，并对MT-W的体内吸收情况进行了检测。结果显示，MT-W在试验设定浓度下，对试验小鼠的体重、器官/体重比率没有显著影响，血液生化指标与对照组也无统计学差异；在按照50 mg/kg体重给实验小鼠灌服MT-W溶液后，在血浆中未检测到MT-W。研究表明，MT-W对小鼠没有明显毒副作用，MT-W在口服后未被有效吸收。

Abstract:

The aim of this experiment was to investigate the toxicity effects of melittin-thanatin recombinant antimicrobial peptide (MT-W) on Kunming mice. The effects of MT-W on major organs of mice were studied by oral acute toxicity test and oral bioavailability test, and biochemical indexes such as alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase in blood were detected, as well as the absorption of MT-W in vivo. The results showed that MT-W had no significant effects on the body weight and organ/body weight ratio of experimental mice, and there was no statistical difference in blood biochemical indexes between the control group and the control group. No MT-W was detected in plasma of mice given MT-W solution at 50mg/kg body weight. Studies have shown that MT-W has no obvious toxic and side effects on mice, and MT-W is not effectively absorbed after oral administration.