不同浓度大豆卵磷脂替代蛋黄对东佛里生奶绵羊精液冷冻保存效果的影响

Effects of Different Concentrations of Soybean Lecithin Instead of Egg Yolk on Cryopreservation of Semen in East Friensian Milk Sheep

摘要:

本试验皆在研究添加不同浓度大豆卵磷脂（SL）冷冻保存东佛里生奶绵羊精液的效果。我们在Tris基础稀释液中，添加18%蛋黄为对照组，添加0.5%、1%、1.5%、2%、2.5%SL设为试验组，检测冷冻精液解冻后的精子活率和顶体完整率。结果显示，添加0.5%、2.5% SL冷冻稀释液稀释的精液，解冻后精子活率和顶体完整率与其他组之间存在显著差异（P<0.05）；添加18%蛋黄和1%～2% SL冷冻稀释液稀释的精液，冷冻解冻后精子活率和顶体完整率之间无显著差异（P>0.05）；添加18%蛋黄和1.0%～1.5% SL冷冻稀释液稀释后的精液，进行人工授精后母羊的妊娠率与对照组无显著差异（P>0.05）。因此，大豆卵磷脂可以作为冷冻保护剂用于东佛里生奶绵羊精液的冷冻保存，其最佳添加浓度为1～2%（g／L）。

Abstract:

The purpose of this experiment was to study the effect of adding different concentrations of soybean lecithin (SL) on the cryopreservation of semen in East Friensian milk sheep. We added 18% egg yolk to the basic dilution of Tris as the control group, and added 0.5%,1%,1.5%,2%,and 2.5% SL as the test group. We measured sperm motility and acrosome integrity after thawing frozen semen. The results showed that there were significant differences(P<0.05) in the sperm viability and acrosome integrity rate between the thawed semen diluted with 0.5% and 2.5% SL freezing diluent and other groups(P<0.05);adding 18% egg yolk and 1%~2% There was no significant difference between the sperm motility rate and acrosome integrity rate in semen diluted with SL frozen diluent after thawing(P>0.05); the semen diluted with 18% egg yolk and 1.0%~1.5% SL frozen diluent, after artificial insemination The pregnancy rate of ewes was not significantly different from that of the control group(P>0.05).Therefore,soybean lecithin can be used as a cryoprotectant for the cryopreservation of semen in East Friensian milk sheep,and the optimum concentration is 1%~2%(g/L).