新版国家标准《新城疫诊断技术》血凝和血凝抑制试验的理解与操作实践

Understanding and Practice of Hemagglutination and Hemagglutination Inhibition Test in the New Version of the National Standard "Newcastle Disease Diagnostic Technology"

摘要:

血凝（HA）和血凝抑制试验（HI）主要是用于新城疫病原鉴定和抗体效价测定，新版《新城疫诊断技术》（GB/T16550-2020）对HA和HI试验判定结果进行了修改和细化，使其更加标准和规范。为尽快理解新版标准要求，熟练掌握操作要领，对新、旧版标准中HA和HI试验进行了比较，新版标准增加了对HA和HI试验4HAU抗原配制的标定、静置时间以及结果判定的改变。新版标准在操作步骤上更为精准详细，4HAU抗原配置更加准确，从而使得HI试验结果更加科学。本文结合实际检测，为广大实验室检验人员、大专院校学生及科研人员更好地理解新版标准，更熟练地操作，从而为提高试验结果准确性提供了参考。

Abstract:

Hemagglutination (HA) and hemagglutination inhibition test (HI) are mainly used for Newcastle disease pathogen identification and antibody titer determination. Revised and refined to make it more standard and normative. In order to understand the requirements of the new version of the standard as soon as possible, and master the essentials of operation, the HA and HI tests in the new and old versions of the standard were compared. The new version of the standard adds the calibration of the 4HAU antigen preparation of the HA and HI tests, and changes in the standing time and the judgment of the results. The new version of the standard is more precise and detailed in the operation steps, and the 4HAU antigen configuration is more accurate, which makes the HI test results more scientific. Combined with the actual test, this paper provides a reference for the majority of laboratory inspectors, college students and scientific researchers to better understand the new version of the standard and operate it more proficiently, thereby improving the accuracy of the test results.