Comparison of Detection Results of 6 Kinds of Diagnostic Reagents for Brucellosis

Abstract:

In order to compare the performance of different brucellosis diagnostic reagents in laboratory tests,In this study,96 samples of cow serum were detected by 4 methods,including rose bengal plate agglutination test (RBT),serum agglutination test (SAT),indirect enzymelinked immunosorbent assay (iELISA) and competitive enzymelinked immunosorbent assay (cELISA),and 6 different reagents.SAT results were used as the gold standard.The sensitivity,specificity and coincidence rate of the other 5 diagnostic reagents were analyzed,and the consistency of the detection results of different reagents with the same method was analyzed.The results showed that 3 positive samples and 93 negative samples were detected by SAT.RBT,iELISAA and iELISAB showed the highest sensitivity (100.00%) compared with SAT.The specificity of RBT and cELISAC was the highest (98.92%).The coincidence rates of RBT,iELISAA and cELISAC were all above 91.67%.The consistency between iELISAA and iELISAB and between cELISAC and cELISAD was poor.The results of this study provided reference for the selection of diagnostic reagents to improve the diagnostic accuracy.