Analysis on the Effect of Double Detection of Antigen and Antibody of Brucellosis in Milk Goats

Abstract:

In order to effectively detect and purify the brucellosis population of dairy goats and accelerate the healthy and steady development of the dairy goat industry, this paper used the dual detection method of brucellosis antigen and antibody to detect and analyze a dairy goat farm in Shaanxi. The results showed that when sheep were introduced into a dairy goat farm, 36 of the 228 sheep in the whole flock were infected by single antibody detection, and the infection rate reached 15.79%. disinfect. Three months later, a wholegroup test was carried out on the remaining 192 sheep, and 18 positive sheep were detected. After the same purification method as last time, the antigen and antibody double detection method was used for the remaining 174 sheep 3 months later. Eight sheep were detected by the brucellosis antibody detection card, and 8 sheep were detected by the brucellosis antigen detection card. 10 sheep, and the numbers of the sheep detected by the brucellosis antibody test card and the brucellosis antigen test card are completely different. Three months later, the remaining 156 sheep were tested by the same method, and only one positive sheep was detected. After 6 months, the remaining 155 sheep were tested by the same method, and no brucellosispositive sheep were found. The use of antigen and antibody double detection continued to detect positive sheep at 3 months and 6 months. The conclusion of this experiment is that compared with the brucellosis detection method with a single antibody, the detection method using both antigen and antibody has a significantly lower positive rate of brucellosis, and can achieve the expected purification effect in a short period of time. This shows that the use of double antibodies is more effective in the detection of sheep brucellosis, and it has more significance for popularization.